منابع مشابه
Chromosome-specific DNA repeat probes.
In research as well as in clinical applications, fluorescence in situ hybridization (FISH) has gained increasing popularity as a highly sensitive technique to study cytogenetic changes. Today, hundreds of commercially available DNA probes serve the basic needs of the biomedical research community. Widespread applications, however, are often limited by the lack of appropriately labeled, specific...
متن کاملProficiency testing for laboratories performing fluorescence in situ hybridization with chromosome-specific DNA probes.
OBJECTIVE To assess laboratory performance, use, and limitations in the joint College of American Pathologists and American College of Medical Genetics proficiency testing program for laboratories performing cytogenetic tests based on fluorescence in situ hybridization (FISH). DATA SOURCES Eight proficiency surveys dealing with FISH detection of microdeletions or microduplications, aneuploidy...
متن کاملFeasibility of in situ hybridisation with chromosome specific DNA probes on paraffin wax embedded tissue.
The feasibility was studied of in situ hybridisation using chromosome specific DNA probes on paraffin wax embedded normal and malignant tissues from different organs. Both isolated nuclei and 5 microns sections were used in in situ hybridisation experiments with biotinylated repetitive DNA probes specific for the centromeric regions of chromosomes 1 and 17. The hybridisation results were visual...
متن کاملRapid and early determination of sex using trophoblast biopsy specimens and Y chromosome specific DNA probes.
The feasibility of determining sex by analysing deoxyribonucleic acid (DNA) with two probes specific for Y chromosomes was shown using DNA obtained from samples of blood from 30 non-related males and females of different ethnic origin. The DNA was spotted on nitrocellulose filters and hybridised with both a repetitive (P1) and a unique (49f) sequence specific for the human Y chromosome. A stron...
متن کاملFast-painting of human metaphase spreads using a chromosome-specific, repeat-depleted DNA library probe.
For chromosome painting, in situ suppression of repetitive DNA sequences has been well established. Such standard protocols usually require large amounts of Cot-I DNA. Recently, it has become possible to deplete repetitive DNA sequences from library probes by magnetic purification and PCR-assisted affinity chromatography. These "repeat-depleted library probes" appear to be extremely useful for ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 2006
ISSN: 0022-1554,1551-5044
DOI: 10.1369/jhc.6a6974.2006